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Rabbit Anti-RTF1 Recombinant Antibody (D7V3W) (CBMAB-CP2321-LY)

The product is antibody recognizes RTF1. The antibody D7V3W immunoassay techniques such as: WB.
See all RTF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey, Hamster, Cattle, S. cerevisiae
Clone
D7V3W
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu364 of human RTF1 protein.
Specificity
Human, Mouse, Rat, Monkey, Hamster, Cattle, S. cerevisiae
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
RTF1 Homolog, Paf1/RNA Polymerase II Complex Component
Introduction
This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA. [provided by RefSeq, Sep 2010]
Entrez Gene ID
Human23168
Mouse76246
Rat366169
Monkey706200
Hamster100773205
Cattle518260
UniProt ID
HumanQ92541
MouseA2AQ19
RatQ4V886
MonkeyF6SCU9
HamsterA0A1U7QF00
CattleF1N6E9
Alternative Names
RTF1 Homolog; Paf1/RNA Polymerase II Complex Component; KIAA0252; Rtf1; Paf1/RNA Polymerase II Complex Component; Homolog (S. Cerevisiae); Rtf1; Paf1/RNA Polymerase II Complex Component; Homolog; RNA Polymerase-Associated Protein RTF1 Homolog; Ortholog Of Mouse Gene Trap Locus 7; GTL7;
Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity).
Biological Process
Biological Process blastocyst growthIEA:Ensembl
Biological Process endodermal cell fate commitmentISS:UniProtKB
Biological Process histone H3-K4 trimethylationIEA:Ensembl
Biological Process negative regulation of transcription by RNA polymerase IIISS:UniProtKB
Biological Process positive regulation of histone H3-K4 methylationIEA:Ensembl
Biological Process stem cell population maintenanceManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process transcription elongation by RNA polymerase II promoterManual Assertion Based On ExperimentIDA:GO_Central
Biological Process Wnt signaling pathwayIEA:UniProtKB-KW
Cellular Location
Nucleus, nucleoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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