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Mouse Anti-SARS-CoV-2 N Recombinant Antibody (CBC02) (CBMAB-CR002LY)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBC002
Application
WB, ICC/IF
Immunogen
The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).
Specificity
SARS Coronavirus, SARS Coronavirus 2
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Concentration
2.25 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
SARS-CoV-2 N
Alternative Names
SARS-CoV Spike, 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (By similarity).
May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Biological Process
Biological Process negative regulation of interferon-beta productionManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process positive regulation of NLRP3 inflammasome complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to host immune responseManual Assertion Based On ExperimentIDA:DisProt
Biological Process viral RNA genome packaging1 PublicationIC:ComplexPortal
Cellular Location
Virion
Host cytoplasm
Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.
PTM
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.
Phosphorylated on serine residues by host GSK3A and GSK3B.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

SARS-CoV-2 N Matched Antibody Pair (NB1) (CAT#: APMAB-1338LY)

SARS-CoV-2 N Matched Antibody Pair (NB2) (CAT#: APMAB-1339LY)

Related Products

Human Anti-SARS-CoV-2 N Monoclonal Antibody (CBFYR-0117) (CAT#: CBMAB-R0117-FY)

Human Anti-SARS-CoV-2 N Recombinant IgM Antibody (M01) (CAT#: CBMAB-CR008LY)

SARS-CoV-2 N Matched Antibody Pair (NB2) (CAT#: APMAB-1339LY)

Human Anti-SARS-CoV-2 N Monoclonal Antibody (CBFYR-0118) (CAT#: CBMAB-R0118-FY)

SARS-CoV-2 N Matched Antibody Pair (NB1) (CAT#: APMAB-1338LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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