Mouse Anti-SEC63 Recombinant Antibody (1A8) (CBMAB-A7959-LY)

Name: Mouse Anti-SEC63 Recombinant Antibody (1A8)
SKU: CBMAB-A7959-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1A8

Application

WB, ELISA

Immunogen

SEC63 (NP_009145.1, 631 a.a. ~ 728 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

SEC63 homolog (S. cerevisiae)

Introduction

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq]

Entrez Gene ID

11231

UniProt ID

Q9UGP8

Alternative Names

ERdj2; PRO2507; SEC63L

Function

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed:22375059, PubMed:29719251).
Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (PubMed:29719251).
Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).

Biological Process

Biological Process liver developmentIEA:Ensembl
Biological Process multicellular organism agingIEA:Ensembl
Biological Process nitrogen compound metabolic processIEA:Ensembl
Biological Process post-translational protein targeting to endoplasmic reticulum membraneManual Assertion Based On ExperimentIMP:MGI
Biological Process post-translational protein targeting to membrane, translocationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein targeting to membraneManual Assertion Based On ExperimentTAS:ProtInc
Biological Process SRP-dependent cotranslational protein targeting to membraneManual Assertion Based On ExperimentIMP:MGI

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Polycystic liver disease 2 with or without kidney cysts (PCLD2):
An autosomal dominant hepatobiliary disease characterized by overgrowth of biliary epithelium and supportive connective tissue, resulting in multiple liver cysts. A subset of patients may develop kidney cysts that usually do not result in clinically significant renal disease.

Topology

Lumenal: 1-14
Helical: 15-35
Cytoplasmic: 36-69
Helical: 70-90
Lumenal: 91-188
Helical: 189-209
Cytoplasmic: 210-760

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-SEC63 Recombinant Antibody (CBXS-3841) (CAT#: CBMAB-S1111-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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