Rabbit Anti-SENP1 Recombinant Antibody (
25H2) (V2LY-0725-LY956)

Name: Rabbit Anti-SENP1 Recombinant Antibody (25H2)
SKU: V2LY-0725-LY956
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-SENP1 Recombinant Antibody (25H2) (V2LY-0725-LY956) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate
All lanes: SENP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 72-95 kDa

Rabbit Anti-SENP1 Recombinant Antibody (25H2) (V2LY-0725-LY956) in IHC

IHC image of V2LY-0725-LY956 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-SENP1 Recombinant Antibody (25H2) (V2LY-0725-LY956) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY956 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 527-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-SENP1 Recombinant Antibody (25H2) (V2LY-0725-LY956) in FC

Overlay Peak curve showing K562 cells stained with V2LY-0725-LY956 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

25H2

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human SENP1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

SUMO1/Sentrin Specific Peptidase 1

Entrez Gene ID

29843

UniProt ID

Q9P0U3

Function

Protease that catalyzes two essential functions in the SUMO pathway (PubMed:10652325, PubMed:15199155, PubMed:16253240, PubMed:16553580, PubMed:21829689, PubMed:21965678, PubMed:23160374, PubMed:24943844, PubMed:25406032, PubMed:29506078).
The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2 (PubMed:16253240).
Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity (PubMed:21829689).
Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity (PubMed:23160374).
Deconjugates SUMO2 from MTA1 (PubMed:21965678).
Deconjugates SUMO1 from METTL3 (PubMed:29506078).
Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed:25406032).
Deconjugates SUMO1 from GPS2 (PubMed:24943844).

Biological Process

Biological Process activation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIGI:MGI
Biological Process protein desumoylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process proteolysisManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus
Cytoplasm
Shuttles between cytoplasm and nucleus.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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