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Mouse Anti-SETDB1 Recombinant Antibody (CBXS-5773) (CBMAB-S0632-CQ)

This product is a mouse antibody that recognizes SETDB1. The antibody CBXS-5773 can be used for immunoassay techniques such as: ICC, IF, WB.
See all SETDB1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBXS-5773
Antibody Isotype
IgG1
Application
ICC, IF, WB

Basic Information

Immunogen
Purified recombinant fragment of human SETDB1 expressed in E. Coli
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascites fluid
Preservative
0.03% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
SET Domain Bifurcated 1
Introduction
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.
Entrez Gene ID
Human9869
Mouse84505
UniProt ID
HumanQ15047
MouseO88974
Alternative Names
SET Domain Bifurcated 1; ERG-Associated Protein With SET Domain; Histone H3-K9 Methyltransferase 4; Lysine N-Methyltransferase 1E; Tudor Domain Containing 21; H3-K9-HMTase 4; EC 2.1.1.43; KMT1E; ESET;
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed:12869583).
Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed:27732843, PubMed:14536086).
Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed:14536086,). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306).
Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306).
In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity).
Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306).
The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
Biological Process
Biological Process heterochromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process histone H3-K9 methylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of single stranded viral RNA replication via double stranded DNA intermediateISS:UniProtKB
Biological Process positive regulation of DNA methylation-dependent heterochromatin assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Ras protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Chromosome
Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
PTM
Degraded by the proteasome, shielded by interaction with ATF7IP.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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