Rabbit Anti-SF3B3 Recombinant Antibody (CBXS-1448) (CBMAB-S4306-CQ)

Name: Rabbit Anti-SF3B3 Recombinant Antibody (CBXS-1448)
SKU: CBMAB-S4306-CQ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Rabbit

Clone

CBXS-1448

Application

WB, IHC-P, FC, IF

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

splicing factor 3b, subunit 3, 130kDa

Introduction

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Entrez Gene ID

Human	23450
Mouse	101943
Rat	292019

UniProt ID

Human	Q15393
Mouse	Q921M3
Rat	B5DF12

Alternative Names

Splicing Factor 3b Subunit 3; Spliceosome-Associated Protein 130; SAP 130; SF3b130; STAF130; SAP130; Pre-MRNA Splicing Factor SF3b, 130 KDa Subunit;

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166).
SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).
May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

Biological Process

Biological Process histone H3 acetylation1 PublicationIC:ComplexPortal
Biological Process monoubiquitinated histone deubiquitinationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process monoubiquitinated histone H2A deubiquitinationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of protein catabolic processManual Assertion Based On ExperimentIDA:CACAO
Biological Process positive regulation of DNA-templated transcription1 PublicationIC:ComplexPortal
Biological Process regulation of DNA repair1 PublicationIC:ComplexPortal
Biological Process regulation of RNA splicing1 PublicationIC:ComplexPortal
Biological Process RNA splicing1 PublicationIC:UniProtKB
Biological Process RNA splicing, via transesterification reactionsManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process U2-type prespliceosome assembly1 PublicationIC:ComplexPortal

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-SF3B3 Recombinant Antibody (CBXS-1449) (CAT#: CBMAB-S4307-CQ)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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