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Rat Anti-SIGLECH Recombinant Antibody (440c) (CBMAB-S0454-CQ)

This product is a rat antibody that recognizes SIGLECH. The antibody 440c can be used for immunoassay techniques such as: FC, FuncS, IHC-Fr.
See all SIGLECH antibodies
Published Data
Fig.1 293T cells expressing murine DAP12 (293T-DAP12) and 293T cells were transiently transfected with a Siglec-H-encoding cDNA, stained with mAb 440c, and analyzed by flow cytometry.
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Fig.2 Depletion efficiency of pDCs after i.p. DT administration in Apoe-/- mice (control) compared to Apoe-/- BDCA2-DTR mice (pDC depleted).
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Fig.3 Representative flow-cytometric profiles, after gating on CD45hi cells, of cDCs (CD11c+ cells) or pDCs (SiglecH+ cells).
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Summary

Host Animal
Rat
Specificity
Mouse
Clone
440c
Antibody Isotype
IgG2b
Application
FC, FuncS, IHC-Fr

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% BSA
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Sialic Acid Binding Ig-Like Lectin H
Entrez Gene ID
233274
UniProt ID
Q4QQK7
Alternative Names
6430529G09Rik; Siglec-H
Biological Process
Biological Process cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process establishment of localization in cellManual Assertion Based On ExperimentIDA:MGI
Biological Process receptor-mediated endocytosisManual Assertion Based On ExperimentIDA:MGI
Cellular Location
cell surface
integral component of membrane
plasma membrane
Topology
Helical: 250-270
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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