Mouse Anti-SIRPA Recombinant Antibody (BA0421) (CBMAB-0839CQ)

Mouse

Human

BA0421

IgG2b

FC

Human

IgG2b

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

>95% as determined by analysis by SDS-PAGE

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

signal regulatory protein alpha

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity).
Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cell migrationISS:ARUK-UCL
Biological Process cellular response to hydrogen peroxideISS:ARUK-UCL
Biological Process cellular response to interferon-gammaISS:ARUK-UCL
Biological Process cellular response to interleukin-1ISS:ARUK-UCL
Biological Process cellular response to interleukin-12Manual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process cellular response to lipopolysaccharideISS:ARUK-UCL
Biological Process monocyte extravasationISS:ARUK-UCL
Biological Process negative regulation of chemokine (C-C motif) ligand 5 productionISS:ARUK-UCL
Biological Process negative regulation of cytokine production involved in inflammatory responseISS:ARUK-UCL
Biological Process negative regulation of ERK1 and ERK2 cascadeISS:ARUK-UCL
Biological Process negative regulation of I-kappaB phosphorylationISS:ARUK-UCL
Biological Process negative regulation of inflammatory responseManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process negative regulation of interferon-beta productionISS:ARUK-UCL
Biological Process negative regulation of interleukin-6 productionISS:ARUK-UCL
Biological Process negative regulation of JNK cascadeISS:ARUK-UCL
Biological Process negative regulation of macrophage inflammatory protein 1 alpha productionISS:ARUK-UCL
Biological Process negative regulation of nitric oxide biosynthetic processISS:ARUK-UCL
Biological Process negative regulation of phagocytosisISS:ARUK-UCL
Biological Process negative regulation of protein phosphorylationISS:ARUK-UCL
Biological Process negative regulation of tumor necrosis factor productionISS:ARUK-UCL
Biological Process positive regulation of phagocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of T cell activationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process regulation of interferon-gamma productionManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process regulation of interleukin-1 beta productionISS:ARUK-UCL
Biological Process regulation of interleukin-6 productionISS:ARUK-UCL
Biological Process regulation of nitric oxide biosynthetic processISS:ARUK-UCL
Biological Process regulation of tumor necrosis factor productionManual Assertion Based On ExperimentIGI:ARUK-UCL

Membrane

Extracellular: 31-373
Helical: 374-394
Cytoplasmic: 395-504

N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.