Mouse Anti-SKA2 Recombinant Antibody (CBXS-2282) (CBMAB-S5044-CQ)

Name: Mouse Anti-SKA2 Recombinant Antibody (CBXS-2282)
SKU: CBMAB-S5044-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBXS-2282

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Spindle And Kinetochore Associated Complex Subunit 2

Entrez Gene ID

Human	348235
Mouse	66140
Rat	287598

UniProt ID

Human	Q8WVK7
Mouse	Q9CR46
Rat	Q5I0J4

Alternative Names

Spindle And Kinetochore Associated Complex Subunit 2; Family With Sequence Similarity 33, Member A; FAM33A; Spindle And Kinetochore-Associated Protein 2; Spindle And KT (Kinetochore) Associated 2; Protein FAM33A;

Function

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020).
Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495).
The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083).
The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083).
In the complex, it is required for SKA1 localization (PubMed:19289083).
Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).

Biological Process

Biological Process cell divisionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of microtubule polymerization or depolymerizationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton, spindle
Chromosome, centromere, kinetochore
Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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