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Rabbit Anti-SKP2 Recombinant Antibody (CBXS-1485) (CBMAB-S4338-CQ)

This product is a rabbit antibody that recognizes SKP2. The antibody CBXS-1485 can be used for immunoassay techniques such as: WB, IHC-P, FC, IF.
See all SKP2 antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
CBXS-1485
Antibody Isotype
IgG
Application
WB, IHC-P, FC, IF

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
S-Phase Kinase Associated Protein 2
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.
Entrez Gene ID
Human6502
Mouse27401
Rat294790
UniProt ID
HumanQ13309
MouseQ9Z0Z3
RatB2GUZ0
Alternative Names
S-Phase Kinase Associated Protein 2; S-Phase Kinase-Associated Protein 2, E3 Ubiquitin Protein Ligase; F-Box/LRR-Repeat Protein 1; P45skp2; FBXL1; S-Phase Kinase-Associated Protein 2 (P45); CDK2/Cyclin A-Associated Protein P45;
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16262255, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22770219, PubMed:32267835).
Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity).
Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1 (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:17962192, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:32267835).
Degradation of TAL1 also requires STUB1 (PubMed:17962192).
Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2 (PubMed:16262255).
Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration (PubMed:22770219).
Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Biological Process
Biological Process defense response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Biological Process G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process innate immune responseManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of intracellular estrogen receptor signaling pathwayIEA:Ensembl
Biological Process positive regulation of protein polyubiquitinationIEA:Ensembl
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of apoptotic processManual Assertion Based On ExperimentIDA:MGI
Biological Process regulation of cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process SCF-dependent proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus
PTM
Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13.
Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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