Mouse Anti-SLC26A5 Recombinant Antibody (CBXS-2782) (CBMAB-S5533-CQ)

Name: Mouse Anti-SLC26A5 Recombinant Antibody (CBXS-2782)
SKU: CBMAB-S5533-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBXS-2782

Application

ELISA, WB

Immunogen

Partial recombinant protein corresponding to aa645-742 from human SLC26A5 (NP_945350) with GST tag

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

solute carrier family 26, member 5 (prestin)

Introduction

This gene encodes a member of the SLC26A/SulP transporter family. The protein functions as a molecular motor in motile outer hair cells (OHCs) of the cochlea, inducing changes in cell length that act to amplify sound levels. The transmembrane protein is an incomplete anion transporter, and does not allow anions to cross the cell membrane but instead undergoes a conformational change in response to changes in intracellular Cl- levels that results in a change in cell length. The protein functions at microsecond rates, which is several orders of magnitude faster than conventional molecular motor proteins. Mutations in this gene are potential candidates for causing neurosensory deafness. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

375611

UniProt ID

P58743

Alternative Names

Solute Carrier Family 26 Member 5; Solute Carrier Family 26 (Anion Exchanger), Member 5; Prestin (Motor Protein); PRES; Deafness, Neurosensory, Autosomal Recessive, 61; Prestin; DFNB61;

Function

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).

Biological Process

Biological Process cochlea developmentIEA:Ensembl
Biological Process fructose transmembrane transportIEA:Ensembl
Biological Process negative regulation of ion transmembrane transportIEA:Ensembl
Biological Process positive regulation of cell motilityIEA:Ensembl
Biological Process positive regulation of cell sizeIEA:Ensembl
Biological Process regulation of cell shapeIEA:UniProtKB-KW
Biological Process regulation of membrane potentialIEA:Ensembl
Biological Process response to auditory stimulusIEA:Ensembl
Biological Process response to ischemiaIEA:Ensembl
Biological Process response to potassium ionIEA:Ensembl
Biological Process response to salicylic acidIEA:Ensembl
Biological Process response to saltIEA:Ensembl
Biological Process response to thyroid hormoneIEA:Ensembl
Biological Process response to xenobiotic stimulusIEA:Ensembl
Biological Process sensory perception of soundManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cell membrane
Lateral wall of outer hair cells.

Involvement in disease

Deafness, autosomal recessive, 61 (DFNB61):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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