Rabbit Anti-SLC2A2 Recombinant Antibody (
37E1) (V2LY-0725-LY1209)

Name: Rabbit Anti-SLC2A2 Recombinant Antibody (37E1)
SKU: V2LY-0725-LY1209
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-SLC2A2 Recombinant Antibody (37E1) (V2LY-0725-LY1209) in IHC

IHC image of V2LY-0725-LY1209 diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.20% DAB.

Rabbit Anti-SLC2A2 Recombinant Antibody (37E1) (V2LY-0725-LY1209) in IF

Immunofluorescence staining of HepG2 with V2LY-0725-LY1209 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 495-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-SLC2A2 Recombinant Antibody (37E1) (V2LY-0725-LY1209) in FC

Overlay Peak curve showing HepG2 cells surface stained with V2LY-0725-LY1209 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

37E1

Application

ELISA, IHC, IF, FC

Immunogen

A synthesized peptide derived from Human SLC2A2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

solute carrier family 2 member 2

Entrez Gene ID

6514

UniProt ID

P11168

Function

Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed:8027028, PubMed:16186102, PubMed:23396969, PubMed:28083649, PubMed:8457197).
Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed:8027028).
May also participate with the Na+/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed:3399500).
Also able to mediate the transport of dehydroascorbate (PubMed:23396969).

Biological Process

Biological Process carbohydrate metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process dehydroascorbic acid transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process fructose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process galactose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process glucose importManual Assertion Based On ExperimentIBA:GO_Central
Biological Process glucose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process monosaccharide transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane

Involvement in disease

Fanconi-Bickel syndrome (FBS):
Rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose.

PTM

N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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