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Mouse Anti-SNAP25 Monoclonal Antibody (SP12) (CBMAB-1472-YC)

Provided herein is a mouse monoclonal antibody against Human SNAP25. The antibody, clone SP12, can be used for immunoassay techniques, such as ELISA, IHC-P and WB.
See all SNAP25 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Hamster, Pig, Rat, Gerbil
Clone
SP12
Antibody Isotype
IgG1
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
Crude human synaptic immunoprecipitate
Specificity
Human, Hamster, Pig, Rat, Gerbil
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Synaptosome Associated Protein 25
Introduction
SNAP25 is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release.
Entrez Gene ID
Human6616
Pig100620588
Rat25012
Hamster100769917
UniProt ID
Alternative Names
SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Biological Process
Biological Process associative learningIEA:Ensembl
Biological Process chemical synaptic transmission1 PublicationNAS:UniProtKB
Biological Process exocytic insertion of neurotransmitter receptor to postsynaptic membraneIEA:Ensembl
Biological Process exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process localization1 PublicationEXP:DisProt
Biological Process locomotory behaviorIEA:Ensembl
Biological Process long-term synaptic potentiationIEA:Ensembl
Biological Process neurotransmitter receptor internalizationIEA:Ensembl
Biological Process neurotransmitter uptake1 PublicationNAS:UniProtKB
Biological Process regulation of insulin secretionManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of neuron projection developmentIEA:Ensembl
Biological Process synaptic vesicle docking1 PublicationNAS:UniProtKB
Biological Process synaptic vesicle exocytosisManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Biological Process synaptic vesicle fusion to presynaptic active zone membraneManual Assertion Based On ExperimentIBA:GO_Central
Biological Process synaptic vesicle primingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process vesicle fusionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm, perinuclear region
Cell membrane
Synapse, synaptosome
Photoreceptor inner segment
Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity).
Colocalizes with PLCL1 at the cell membrane (By similarity).
Involvement in disease
Myasthenic syndrome, congenital, 18 (CMS18):
A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. CMS18 is an autosomal dominant presynaptic disorder clinically characterized by early-onset muscle weakness and easy fatigability associated with delayed psychomotor development and ataxia.
PTM
Palmitoylated (PubMed:28757145).
Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:15592454, PubMed:9886085).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release (PubMed:9886085, PubMed:17718519).
C.botulinum type C only rarely infects humans.
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:9886085).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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