Mouse Anti-SNRPD1 Recombinant Antibody (
24E2) (V2LY-0725-LY882)

Name: Mouse Anti-SNRPD1 Recombinant Antibody (24E2)
SKU: V2LY-0725-LY882
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-SNRPD1 Recombinant Antibody (24E2) (V2LY-0725-LY882) in IHC

IHC image of V2LY-0725-LY882 diluted at 1:50 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-SNRPD1 Recombinant Antibody (24E2) (V2LY-0725-LY882) in IHC

IHC image of V2LY-0725-LY882 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-SNRPD1 Recombinant Antibody (24E2) (V2LY-0725-LY882) in IF

Immunofluorescence staining of A549 cell with V2LY-0725-LY882 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-SNRPD1 Recombinant Antibody (24E2) (V2LY-0725-LY882) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY882 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

24E2

Application

ELISA, IHC, IF, FC

Immunogen

Recombinant Human SNRPD1 protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:20-1:200
IF	1:20-1:200
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Small Nuclear Ribonucleoprotein D1 Polypeptide

Entrez Gene ID

6632

UniProt ID

P62314

Function

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:23333303, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346).
Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346).
Is also a component of the minor U12 spliceosome (PubMed:15146077).
May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (Probable)

Biological Process

Biological Process 7-methylguanosine cap hypermethylation1 PublicationIC:ComplexPortal
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process spliceosomal complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Biological Process spliceosomal snRNP assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process U2-type prespliceosome assembly1 PublicationIC:ComplexPortal

Cellular Location

Cytoplasm, cytosol
Nucleus
SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.

PTM

Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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