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Rabbit Anti-SPDL1 Recombinant Antibody (EG508) (CBMAB-EN576-LY)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
EG508
Application
WB: 1:500~1:1000 ELISA: 1:40000
Immunogen
The antibody was produced against synthesized peptide derived from C-terminal of human CCDC99.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Spindle Apparatus Coiled-Coil Protein 1
Introduction
This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]
Entrez Gene ID
54908
UniProt ID
Q96EA4
Alternative Names
Spindle Apparatus Coiled-Coil Protein 1; Coiled-Coil Domain-Containing Protein 99; Rhabdomyosarcoma Antigen MU-RMS-40.4A; Arsenite-Related Gene 1 Protein; CCDC99; Spindle Apparatus Coiled-Coil Domain-Containing Protein 1;
Function
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067).
Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).
Plays a role in cell migration (PubMed:30258100).
Biological Process
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process establishment of mitotic spindle orientationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic spindle assembly checkpoint signalingIEA:InterPro
Biological Process protein localization to kinetochoreManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Chromosome, centromere, kinetochore
Nucleus
Cytoplasm, cytoskeleton, spindle pole
Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
PTM
Monoubiquitinated with'Lys-48' linkage (PubMed:30258100).
Deubiquitinated by USP45 (PubMed:30258100).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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