SPDL1

This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]

Full Name

Spindle Apparatus Coiled-Coil Protein 1

Function

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067).
Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).
Plays a role in cell migration (PubMed:30258100).

Biological Process

Biological Process cell divisionIEA:UniProtKB-KW
Biological Process cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process establishment of mitotic spindle orientationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic spindle assembly checkpoint signalingIEA:InterPro
Biological Process protein localization to kinetochoreManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Chromosome, centromere, kinetochore
Nucleus
Cytoplasm, cytoskeleton, spindle pole
Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.

PTM

Monoubiquitinated with'Lys-48' linkage (PubMed:30258100).
Deubiquitinated by USP45 (PubMed:30258100).