Recombinant Mouse Anti-SPRED1 Recombinant Antibody (M23-P2G3) (CBMAB-XB1064-YC)

Name: Recombinant Mouse Anti-SPRED1 Recombinant Antibody (M23-P2G3)
SKU: CBMAB-XB1064-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

M23-P2G3

Application

ELISA, IF, WB

Immunogen

Peptide sequence YVPLRMCHR (amino acids 420-428)

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Sprouty Related EVH1 Domain Containing 1

Introduction

SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).

Entrez Gene ID

161742

UniProt ID

Q7Z699

Alternative Names

Sprouty Related EVH1 Domain Containing 1; Protein Phosphatase 1, Regulatory Subunit 147; Spred-1; HSpred1; Sprouty-Related, EVH1 Domain-Containing Protein 1; Suppressor Of Ras/MAPK Activation; PPP1R147; NFLS;

Function

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity).
Negatively regulates hematopoiesis of bone marrow (By similarity).
Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity).
Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:18216281).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity).

Biological Process

Biological Process negative regulation of angiogenesisManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of cell migration involved in sprouting angiogenesisIEA:Ensembl
Biological Process negative regulation of epithelial to mesenchymal transitionISS:UniProtKB
Biological Process negative regulation of ERK1 and ERK2 cascadeISS:UniProtKB
Biological Process negative regulation of lens fiber cell differentiationISS:UniProtKB
Biological Process negative regulation of MAPK cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of protein kinase activityManual Assertion Based On ExperimentIDA:ARUK-UCL
Biological Process negative regulation of transforming growth factor beta receptor signaling pathwayISS:UniProtKB
Biological Process positive regulation of DNA damage response, signal transduction by p53 class mediatorBy SimilarityISS:BHF-UCL
Biological Process regulation of MAPK cascadeManual Assertion Based On ExperimentTAS:ARUK-UCL
Biological Process regulation of protein deacetylationBy SimilarityISS:BHF-UCL
Biological Process vasculogenesis involved in coronary vascular morphogenesisManual Assertion Based On ExperimentIMP:BHF-UCL

Cellular Location

Cell membrane
Membrane, caveola
Nucleus
Localized in cholesterol-rich membrane raft/caveola fractions.

Involvement in disease

Legius syndrome (LGSS):
An autosomal dominant syndrome characterized mainly by cafe-au-lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities, and features of attention-deficit hyperactivity disorder.

PTM

Palmitoylated by ZDHHC17/HIP14.
Phosphorylated on tyrosine.
Ubiquitinated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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