Rabbit Anti-SPRY4 Recombinant Antibody (CBXS-1307) (CBMAB-S4166-CQ)

Name: Rabbit Anti-SPRY4 Recombinant Antibody (CBXS-1307)
SKU: CBMAB-S4166-CQ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Rabbit

Clone

CBXS-1307

Application

WB, IP, FC, IF

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Sprouty RTK Signaling Antagonist 4

Introduction

This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants.

Entrez Gene ID

Human	81848
Mouse	24066
Rat	291610

UniProt ID

Human	Q9C004
Mouse	Q91WK1
Rat	D3ZJA3

Alternative Names

Sprouty RTK Signaling Antagonist 4; Sprouty Homolog 4 (Drosophila); Protein Sprouty Homolog 4; Sprouty Homolog 4; Spry-4; HH17;

Function

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893).
Probably impairs the formation of GTP-Ras (PubMed:12027893).
Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443).
Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).

Biological Process

Biological Process animal organ developmentManual Assertion Based On ExperimentIBA:GO_Central
Biological Process cellular response to leukemia inhibitory factorIEA:Ensembl
Biological Process negative regulation of ERK1 and ERK2 cascadeIEA:InterPro
Biological Process negative regulation of fibroblast growth factor receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of MAP kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of Ras protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm
Cell projection, ruffle membrane
Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity).
Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898).

Involvement in disease

Hypogonadotropic hypogonadism 17 with or without anosmia (HH17):
A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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