Mouse Anti-SUPT16H Recombinant Antibody (1F1) (CBMAB-A8780-LY)

Name: Mouse Anti-SUPT16H Recombinant Antibody (1F1)
SKU: CBMAB-A8780-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1F1

Application

WB, ELISA

Immunogen

SUPT16H (NP_009123, 608 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

SUPT16H

Introduction

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq]

Entrez Gene ID

11198

UniProt ID

Q9Y5B9

Alternative Names

CDC68; FACT; FACTP140; FLJ10857; FLJ14010; FLJ34357; SPT16/CDC68

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).

Biological Process

Biological Process DNA repairIEA:UniProtKB-KW
Biological Process DNA replicationIEA:UniProtKB-KW
Biological Process DNA replication-independent chromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process nucleosome assembly1 PublicationIC:ComplexPortal
Biological Process nucleosome disassemblyManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process positive regulation of DNA-templated transcription, elongationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process positive regulation of transcription elongation by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Biological Process transcription elongation by RNA polymerase II promoterManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Chromosome
Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.

Involvement in disease

Neurodevelopmental disorder with dysmorphic facies and thin corpus callosum (NEDDFAC):
An autosomal dominant disorder characterized by global developmental delay, impaired intellectual development with poor or absent speech and language, and autistic-like behaviors. Corpus callosum anomalies are visible on brain imaging. Most patients have dysmorphic features including tall forehead, down-slanting palpebral fissures, ear anomalies and broad nasal bridge. Other variably present clinical features include seizures, sleeping difficulties and precocious puberty.

PTM

ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

SUPT16H Matched Antibody Pair (1171) (CAT#: APMAB-1171LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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