SUPT16H

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq]

SUPT16H

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).

Biological Process DNA repairIEA:UniProtKB-KW
Biological Process DNA replicationIEA:UniProtKB-KW
Biological Process DNA replication-independent chromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process nucleosome assembly1 PublicationIC:ComplexPortal
Biological Process nucleosome disassemblyManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process positive regulation of DNA-templated transcription, elongationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process positive regulation of transcription elongation by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Biological Process transcription elongation by RNA polymerase II promoterManual Assertion Based On ExperimentIBA:GO_Central

Nucleus
Chromosome
Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.

Neurodevelopmental disorder with dysmorphic facies and thin corpus callosum (NEDDFAC):
An autosomal dominant disorder characterized by global developmental delay, impaired intellectual development with poor or absent speech and language, and autistic-like behaviors. Corpus callosum anomalies are visible on brain imaging. Most patients have dysmorphic features including tall forehead, down-slanting palpebral fissures, ear anomalies and broad nasal bridge. Other variably present clinical features include seizures, sleeping difficulties and precocious puberty.

ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.