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Mouse Anti-SYNJ2 Recombinant Antibody (2B11) (CBMAB-A8812-LY)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
2B11
Application
WB, ELISA
Immunogen
SYNJ2 (NP_003889, 1396 a.a. ~ 1495 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
SYNJ2
Introduction
Synaptojanin-2 is a ubiquitously expressed inositol polyphosphate 5-phosphatase (Malecz et al., 2000 [PubMed 11084340]).[supplied by OMIM
Entrez Gene ID
8871
UniProt ID
O15056
Alternative Names
INPP5H; KIAA0348; MGC44422
Function
Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis.
Biological Process
Biological Process brain developmentIBA:GO_Central1 Publication
Biological Process inositol phosphate dephosphorylationIBA:GO_Central1 Publication
Biological Process membrane organizationTAS:Reactome
Biological Process phosphatidylinositol biosynthetic processTAS:Reactome
Biological Process phosphatidylinositol dephosphorylationIBA:GO_Central1 Publication
Biological Process synaptic vesicle endocytosisIBA:GO_Central1 Publication
Cellular Location
Cytoplasm
Cell membrane
Membrane raft
Presynapse
Cytoplasm, cytoskeleton
Localizes at presynapse terminals in brain and at bundles of microtubules surrounding the nucleus in the elongating spermatids corresponding to the manchette (By similarity).
Translocates from the cytoplasm to membrane ruffles in a RAC1-dependent manner (PubMed:11084340).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

SYNJ2 Matched Antibody Pair (1175) (CAT#: APMAB-1175LY)

Related Products

Mouse Anti-SYNJ2 Recombinant Antibody (2H8) (CAT#: CBMAB-A8813-LY)

Rabbit Anti-SYNJ2 Recombinant Antibody (CBXS-1456) (CAT#: CBMAB-S4313-CQ)

SYNJ2 Matched Antibody Pair (1175) (CAT#: APMAB-1175LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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