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Mouse Anti-TAF12 Recombinant Antibody (1E10) (CBMAB-A8844-LY)

The product is antibody recognizes TAF12. The antibody 1E10 immunoassay techniques such as: WB, ELISA.
See all TAF12 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E10
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
TAF12 (AAH11986, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
Introduction
Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Entrez Gene ID
6883
UniProt ID
Q16514
Alternative Names
TAF2J; TAFII20
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473).
Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:10373431, PubMed:7729427, PubMed:8598932, PubMed:8663456, PubMed:9674425, PubMed:9885574).
Biological Process
Biological Process DNA-templated transcription initiationIDA:UniProtKB1 Publication
Biological Process histone acetylationIC:ComplexPortal1 Publication
Biological Process histone H3 acetylationIDA:UniProtKB1 Publication
Biological Process monoubiquitinated histone deubiquitinationIDA:ComplexPortal1 Publication
Biological Process monoubiquitinated histone H2A deubiquitinationIDA:ComplexPortal1 Publication
Biological Process mRNA transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process positive regulation of DNA-binding transcription factor activityIDA:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionIC:ComplexPortal1 Publication
Biological Process positive regulation of transcription by RNA polymerase IIIDA:UniProtKB1 Publication
Biological Process positive regulation of transcription initiation by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process protein phosphorylationIDA:ComplexPortal1 Publication
Biological Process regulation of DNA repairIC:ComplexPortal2 Publications
Biological Process regulation of DNA-templated transcriptionIC:ComplexPortal1 Publication
Biological Process regulation of RNA splicingIC:ComplexPortal1 Publication
Biological Process regulation of transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process RNA polymerase II preinitiation complex assemblyIPI:ComplexPortal1 Publication
Biological Process transcription by RNA polymerase IITAS:ProtInc1 Publication
Biological Process transcription initiation at RNA polymerase II promoterIDA:UniProtKB1 Publication
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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