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Mouse Anti-PMEPA1 Recombinant Antibody (2A12) (CBMAB-A9219-LY)

The product is antibody recognizes TMEPAI. The antibody 2A12 immunoassay techniques such as: WB, ELISA.
See all PMEPA1 antibodies
Published Data
Fig.1 A549 cells were treated with endocytosis inhibitor dynasore or genestein before being fixed and double labelled with antibodies to TMEPAI.
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Fig.2 Clathrin regulates TMEPAI intracellular localization.
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Fig.3 A549 cell lines were extracted and immunoprecipitated with antibody against Flag-tag, and the immunoprecipitates were analyzed by Western blot with antibody to Flag and TMEPAI.
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Fig.4 Immunofluorescence microscopy of A549 cell lines labelled with antibodies against TMEPAI.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2A12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
TMEPAI (AAH15918, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Prostate Transmembrane Protein, Androgen Induced 1
Entrez Gene ID
56937
UniProt ID
Q969W9
Alternative Names
STAG1; TMEPAI
Function
Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed:20129061, PubMed:24627487).
Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4 (PubMed:18703514).
Biological Process
Androgen receptor signaling pathway1 PublicationNAS:UniProtKB
Negative regulation of pathway-restricted SMAD protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of SMAD protein complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Early endosome membrane
Golgi apparatus membrane
Topology
Lumenal: 1-40
Helical: 41-63
Cytoplasmic: 64-287
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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