Mouse Anti-PMEPA1 Recombinant Antibody (2A12) (CBMAB-P2221-YC)

Name: Mouse Anti-PMEPA1 Recombinant Antibody (2A12)
SKU: CBMAB-P2221-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

2A12

Application

ELISA, WB

Immunogen

TMEPAI (AAH15918, 181-280 aa) partial recombinant protein with GST tag. Immunogen sequence: NRTIFDSDLM DSARLGGPCP PSSNSGISAT CYGSGGRMEG PPPTYSEVIG HYPGSSFQHQ QSSGPPSLLE GTRLHHTHIA PLESAAIWSK EKDKQKGHPL

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 181-280

More Infomation

Target

Full Name

Prostate Transmembrane Protein, Androgen Induced 1

Introduction

PMEPA1 is a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins.

Entrez Gene ID

56937

UniProt ID

Q969W9

Alternative Names

TMEPAI; STAG1

Function

Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed:20129061, PubMed:24627487).
Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4 (PubMed:18703514).

Biological Process

Androgen receptor signaling pathway1 PublicationNAS:UniProtKB
Negative regulation of pathway-restricted SMAD protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of SMAD protein complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Early endosome membrane
Golgi apparatus membrane

Topology

Lumenal: 1-40
Helical: 41-63
Cytoplasmic: 64-287

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-PMEPA1 Recombinant Antibody (2A12) (CAT#: CBMAB-A9219-LY)

Related Products

Mouse Anti-PMEPA1 Recombinant Antibody (2A12) (CAT#: CBMAB-A9219-LY)

Isotype control

For research use only. Not intended for any clinical use.

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