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Mouse Anti-TNKS Recombinant Antibody (V2-620734) (CBMAB-A9396-LY)

The product is antibody recognizes TNKS. The antibody 1A2 immunoassay techniques such as: WB, ELISA.
See all TNKS antibodies
Published Data
Fig.1 IHC evaluation of TNKS1/2 proteins in human HCC tumors (T) compared to matched adjacent non-tumor liver (nT).
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Fig.2 Tankyrase inhibition stabilizes AXIN levels and decreases β-catenin in human HCC cell lines.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-620734
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
TNKS (NP_003738, 1017 a.a. ~ 1124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Entrez Gene ID
8658
UniProt ID
O95271
Alternative Names
PARP-5a; PARP5A; PARPL; TIN1; TINF1; TNKS1
Function
Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379).
Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859).
Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859).
Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745).
Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114).
May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299).
May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287).
Stimulates 26S proteasome activity (PubMed:23622245).
Biological Process
Biological Process cell division Source:UniProtKB-KW
Biological Process mitotic spindle organization Source:BHF-UCL1 Publication
Biological Process mRNA transport Source:UniProtKB-KW
Biological Process negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric Source:BHF-UCL1 Publication
Biological Process negative regulation of telomere maintenance via telomere lengthening Source:BHF-UCL1 Publication
Biological Process negative regulation of telomeric DNA binding Source:BHF-UCL1 Publication
Biological Process peptidyl-serine phosphorylation Source:MGI1 Publication
Biological Process peptidyl-threonine phosphorylation Source:MGI1 Publication
Biological Process positive regulation of canonical Wnt signaling pathway Source:UniProtKB1 Publication
Biological Process positive regulation of telomerase activity Source:BHF-UCL1 Publication
Biological Process positive regulation of telomere capping Source:BHF-UCL1 Publication
Biological Process positive regulation of telomere maintenance via telomerase Source:BHF-UCL2 Publications
Biological Process positive regulation of transcription by RNA polymerase II Source:UniProtKB1 Publication
Biological Process protein ADP-ribosylation Source:BHF-UCL2 Publications
Biological Process protein auto-ADP-ribosylation Source:UniProtKB2 Publications
Biological Process protein localization to chromosome, telomeric region Source:BHF-UCL2 Publications
Biological Process protein poly-ADP-ribosylation Source:UniProtKB2 Publications
Biological Process protein polyubiquitination Source:UniProtKB2 Publications
Biological Process protein transport Source:UniProtKB-KW
Biological Process regulation of telomere maintenance via telomerase Source:BHF-UCL1 Publication
Biological Process spindle assembly Source:BHF-UCL1 Publication
Biological Process Wnt signaling pathway Source:UniProtKB-KW
Cellular Location
Cytoplasm
Golgi apparatus membrane
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Nucleus, nuclear pore complex
Chromosome, telomere
Cytoplasm, cytoskeleton, spindle pole
Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles (PubMed:22864114).
A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres (PubMed:10523501).
During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1 (PubMed:12768206).
Localizes to spindle poles at mitosis onset via interaction with NUMA1 (PubMed:12080061).
PTM
Phosphorylated on serine residues by MAPK kinases upon insulin stimulation. Phosphorylated during mitosis.
Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation.
ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
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For research use only. Not intended for any clinical use.

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