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Mouse Anti-TOP1MT Recombinant Antibody (TPIMT-1) (CBMAB-T3656-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TOP1MT (DNA Topoisomerase I Mitochondrial). The antibody can be used for immunoassay techniques, such as IF, IHC-P.
See all TOP1MT antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
TPIMT-1
Antibody Isotype
IgG2b, κ
Application
IF, IHC-P

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
0.01% BSA
Preservative
0.05% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Topoisomerase I Mitochondrial
Introduction
TOP1MT is a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. TOP1MT is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription.
Entrez Gene ID
116447
UniProt ID
Q969P6
Alternative Names
DNA Topoisomerase I Mitochondrial; EC 5.99.1.2; Topoisomerase (DNA) I, Mitochondrial; TOP1mt
Function
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Biological Process
Biological Process DNA replication Source:GO_Central1 Publication
Biological Process DNA topological change Source:GO_Central1 Publication
Cellular Location
Mitochondrion
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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