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Mouse Anti-TOP3B Recombinant Antibody (CBYJT-4217) (CBMAB-T3681-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TOP3B (DNA Topoisomerase III Beta). The antibody can be used for immunoassay techniques, such as IF, ELISA, WB.
See all TOP3B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4217
Antibody Isotype
IgG2a, κ
Application
IF, ELISA, WB

Basic Information

Immunogen
TOP3B (AAH02432, 1 a.a. ~ 862 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-862

Target

Full Name
TOP3B Gene(Protein Coding) DNA Topoisomerase III Beta
Introduction
TOP3B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. TOP3B catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. TOP3B interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of TOP3B may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22.
Entrez Gene ID
8940
UniProt ID
O95985
Alternative Names
DNA Topoisomerase III Beta; DNA Topoisomerase III Beta-1; TOP3B1; Topoisomerase (DNA) III Beta; DNA Topoisomerase 3-Beta-1; Topoisomerase III Beta; EC 5.99.1.2
Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Possesses negatively supercoiled DNA relaxing activity.
Biological Process
Biological Process chromosome segregation Source:Ensembl
Biological Process DNA topological change Source:GO_Central1 Publication
Cellular Location
condensed chromosome
DNA topoisomerase III-beta-TDRD3 complex
nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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