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Mouse Anti-TPMT Recombinant Antibody (CBYJT-4358) (CBMAB-T3846-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TPMT (Thiopurine S-Methyltransferase). The antibody can be used for immunoassay techniques, such as ICC, IF, IHC, IHC-P, WB.
See all TPMT antibodies
Published Data
Fig.1 Flow cytometry histograms of green fluorescence of selected stable clones expressing recombinant EGFP–TPMT protein.
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Fig.2 Immunodetection of recombinant EGFP–TPMT.
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Fig.3 Green fluorescence of HEK 293 clones stably expressing the recombinant EGFP–TPMT.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4358
Antibody Isotype
IgG1
Application
ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.3, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
thiopurine S-methyltransferase
Introduction
TPMT is the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency.
Entrez Gene ID
7172
UniProt ID
P51580
Alternative Names
Thiopurine S-Methyltransferase; EC 2.1.1.67; S-Adenosyl-L-Methionine:Thiopurine S-Methyltransferase; Thiopurine Methyltransferase; TPMTD
Function
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed:657528, PubMed:18484748).
TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
Biological Process
Biological Process methylation Source:Reactome
Biological Process nucleobase-containing compound metabolic process Source:ProtInc1 Publication
Biological Process xenobiotic catabolic process Source:Reactome
Biological Process xenobiotic metabolic process Source:UniProtKB2 Publications
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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