Sign in or Register   Sign in or Register
  |  

Mouse Anti-TXK Recombinant Antibody (2E4) (CBMAB-A9670-LY)

The product is antibody recognizes TXK. The antibody 2E4 immunoassay techniques such as: WB, ELISA.
See all TXK antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
TXK (NP_003319, 131 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TXK tyrosine kinase
Entrez Gene ID
7294
UniProt ID
P42681
Alternative Names
BTKL; MGC22473; PSCTK5; PTK4; RLK; TKL
Function
Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation leads to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with PARP1 and EEF1A1 (PubMed:11859127, PubMed:17177976).
Within the complex, phosphorylates both PARP1 and EEF1A1 (PubMed:17177976).
Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
Biological Process
Biological Process activation of phospholipase C activity Source:UniProtKB
Biological Process adaptive immune response Source:UniProtKB
Biological Process integrin-mediated signaling pathway Source:GO_Central1 Publication
Biological Process positive regulation of cytokine production Source:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase II Source:NTNU_SB1 Publication
Biological Process positive regulation of type II interferon production Source:UniProtKB2 Publications
Biological Process positive regulation of type II interferon-mediated signaling pathway Source:UniProtKB1 Publication
Biological Process protein autophosphorylation Source:UniProtKB2 Publications
Biological Process protein phosphorylation Source:UniProtKB1 Publication
Biological Process regulation of gene expression Source:GO_Central1 Publication
Biological Process regulation of platelet activation Source:GO_Central1 Publication
Biological Process T cell receptor signaling pathway Source:UniProtKB
Biological Process tissue regeneration Source:GO_Central1 Publication
Cellular Location
Cytoplasm
Nucleus
Cell membrane
Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.
PTM
Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription.
The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-TXK Recombinant Antibody (2E4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare