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Mouse Anti-TXN Recombinant Antibody (2A7) (CBMAB-A9675-LY)

The product is antibody recognizes TXN. The antibody 2A7 immunoassay techniques such as: WB, ELISA.
See all TXN antibodies
Published Data
Fig.1 Representative western blot of Prdx1, Trx1, Srx1 and Sod1 expression in the erythrocyte cell lysate of beta-thalassemia intermedia patients compared to healthy individuals.
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Fig.2 Characterization of esophageal cell lines for Trx, TXNIP and TXNRD.
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Fig.3 TXNIP is increased in OE21 cells after MS-275/AZA treatment.
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Fig.4 Western blot analysis of YAP, Nrf2, HO-1, Trx, and GSTA4 expressions in 253J, 253J C-r, 253J B-V and 253 J B-V C-r cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2A7
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
TXN (AAH03377, 1 a.a. ~ 105 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
TXN Gene(Protein Coding) Thioredoxin
Introduction
Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM
Entrez Gene ID
7295
UniProt ID
P10599
Alternative Names
DKFZp686B1993; MGC61975; TRX; TRX1
Function
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:2176490, PubMed:17182577, PubMed:19032234).
Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900).
Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:9108029, PubMed:11118054).
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Biological Process
Biological Process activation of protein kinase B activity Source:ParkinsonsUK-UCL1 Publication
Biological Process cell redox homeostasis Source:UniProtKB1 Publication
Biological Process negative regulation of hydrogen peroxide-induced cell death Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of protein export from nucleus Source:Ensembl
Biological Process negative regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process positive regulation of DNA binding Source:UniProtKB1 Publication
Biological Process positive regulation of peptidyl-cysteine S-nitrosylation Source:UniProtKB3 Publications
Biological Process positive regulation of peptidyl-serine phosphorylation Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of protein kinase B signaling Source:ParkinsonsUK-UCL1 Publication
Biological Process response to nitric oxide Source:UniProtKB2 Publications
Biological Process response to radiation Source:UniProtKB2 Publications
Cellular Location
Nucleus
Cytoplasm
Secreted
Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029).
Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054).
Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054).
Secreted by a leaderless secretory pathway (PubMed:1332947).
Involvement in disease
Causes an allergic reaction in human (PubMed:17182577, PubMed:19032234, PubMed:21489611).
Recombinant protein binds to IgE in atopic eczema-suffering patients allergic to opportunistic skin-colonizing yeast M.sympodialis. Intermediate cross-reactivity is detected between the recombinant thioredoxin from M.sympodialis (Mala s 13) and recombinant form of this protein. Skin-prick test (SPT) and atopy patch test (APT) with 5 patients confirm cross-reactivity between the two proteins (PubMed:17182577).
Recombinant protein binds to IgE of patients suffering from allergic bronchopulmonary aspergillosis (ABPA) and cross-reacts extensively with recombinant thioredoxin proteins from A.fumigatus (Asp f 28 and Asp f 29) and Mala s 13. Causes a positive skin reaction and induces proliferation of the human peripheral blood mononuclear cells in ABPA patients allergic to this protein. Acts as an IgE-binding self-antigen in ABPA patients allergic to fungal thioredoxin (PubMed:19032234).
In atopic dermatitis (AD)-suffering patients allergic to M.sympodialis, a cross-reactivity between Mala s 13 and this protein can be detected in T-cells of the peripheral blood and skin. Keratinocytes stimulated by interferon (IFN)-alpha and tumor necrosis factor (TNF)-alpha release thioredoxin, which then becomes available for cross-reactivity with Mala s 13-specific T cells. The autoreactive T cells identified include T-helper 1 (Th1), T-helper 2 (Th2), T-helper 17 (Th17) and T-helper 22 (Th22) phenotypes. Skinhoming T cells autoreactive to this protein may be relevant for cutaneous inflammation in patients with AD (PubMed:21489611).
PTM
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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