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Mouse Anti-U2AF2 Recombinant Antibody (5G8) (CBMAB-A9691-LY)

The product is antibody recognizes U2AF2. The antibody 5G8 immunoassay techniques such as: WB, ELISA.
See all U2AF2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5G8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
U2AF2 (NP_001012496.1, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
U2 small nuclear RNA auxiliary factor 2
Introduction
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]
Entrez Gene ID
11338
UniProt ID
P26368
Alternative Names
U2AF65
Function
Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186).
By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736).
Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).
Biological Process
Biological Process mRNA processing Source:ProtInc1 Publication
Biological Process mRNA splicing, via spliceosome Source:GO_Central1 Publication
Biological Process negative regulation of mRNA splicing, via spliceosome Source:UniProtKB1 Publication
Biological Process negative regulation of protein ubiquitination Source:BHF-UCL1 Publication
Biological Process positive regulation of RNA splicing Source:UniProtKB1 Publication
Cellular Location
Nucleus
PTM
Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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