Mouse Anti-U2AF2 Recombinant Antibody (CBFYU-008) (CBMAB-U0008-FY)

Name: Mouse Anti-U2AF2 Recombinant Antibody (CBFYU-008)
SKU: CBMAB-U0008-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYU-008

Application

ELISA, IF, WB

Immunogen

U2AF2 (NP_001012496.1, 141aa-240aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 kD.

Specificity

Human

Antibody Isotype

IgG1, к

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 141-240

More Infomation

Target

Full Name

U2 small nuclear RNA auxiliary factor 2

Introduction

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Entrez Gene ID

11338

UniProt ID

P26368

Alternative Names

U2AF65

Function

Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186).
By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736).
Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).

Biological Process

Biological Process mRNA processing Source:ProtInc1 Publication
Biological Process mRNA splicing, via spliceosome Source:GO_Central1 Publication
Biological Process negative regulation of mRNA splicing, via spliceosome Source:UniProtKB1 Publication
Biological Process negative regulation of protein ubiquitination Source:BHF-UCL1 Publication
Biological Process positive regulation of RNA splicing Source:UniProtKB1 Publication

Cellular Location

Nucleus

PTM

Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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