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Rabbit Anti-UBE2F Recombinant Antibody (CBWJN-1700) (CBMAB-N4349-WJ)

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Basic Information

Host Animal
Rabbit
Clone
CBWJN-1700
Application
WB, IHC-P, FC, ICC
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
0.825 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Ubiquitin Conjugating Enzyme E2 F (Putative)
Introduction
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Entrez Gene ID
140739
UniProt ID
Q969M7
Alternative Names
Ubiquitin Conjugating Enzyme E2 F (Putative); Ubiquitin-Conjugating Enzyme E2F (Putative); RING-Type E3 NEDD8 Transferase UBE2F; NEDD8 Carrier Protein UBE2F; NEDD8 Protein Ligase UBE2F; NEDD8-Conjugating Enzyme 2; NCE2;
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Biological Process
Biological Process post-translational protein modification Source:Reactome
Biological Process protein neddylation Source:UniProtKB1 Publication
Cellular Location
cytosol
nucleus
PTM
The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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