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Mouse Anti-UBE2H Recombinant Antibody (3C4-1A2) (CBMAB-U0111-FY)

This product is mouse antibody that recognizes UBE2H. The antibody 3C4-1A2 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all UBE2H antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3C4-1A2
Antibody Isotype
IgG2a, к
Application
ELISA, IF, WB

Basic Information

Immunogen
Full length recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL.
Specificity
Human
Antibody Isotype
IgG2a, к
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-183

Target

Full Name
ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Introduction
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
Entrez Gene ID
UniProt ID
Alternative Names
GID3; UBC8; UBCH; UBCH2; E2-20K
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386).
E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972).
In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386).
Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).
Biological Process
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:Ensembl
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication
Cellular Location
cytosol
nucleus
PTM
Autoubiquitinated in vitro in the presence of NEDD4L.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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