Mouse Anti-UBE2H Recombinant Antibody (3C4-1A2) (CBMAB-U0111-FY)

Name: Mouse Anti-UBE2H Recombinant Antibody (3C4-1A2)
SKU: CBMAB-U0111-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

3C4-1A2

Application

ELISA, IF, WB

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL.

Specificity

Human

Antibody Isotype

IgG2a, к

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-183

More Infomation

Target

Full Name

ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)

Introduction

This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Entrez Gene ID

7328

UniProt ID

P62256

Alternative Names

GID3; UBC8; UBCH; UBCH2; E2-20K

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386).
E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972).
In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386).
Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).

Biological Process

Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:Ensembl
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication

Cellular Location

cytosol
nucleus

PTM

Autoubiquitinated in vitro in the presence of NEDD4L.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-UBE2H Recombinant Antibody (CBFYU-112) (CAT#: CBMAB-U0112-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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