Human Recombinant UBE2H protein (V2LY-0526-LY7498)

Name: Human Recombinant UBE2H protein
SKU: V2LY-0526-LY7498
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant UBE2H protein consist of Amino Acid: 1-183 and predicts a molecular mass of 21 kDa.

Molecule Mass

21 kDa

Sequence

Amino Acid: 1-183

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>93% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386).
E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972).
In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386).
Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).

Biological Process

Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:Ensembl
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication

Cellular Location

cytosol
nucleus

PTM

Autoubiquitinated in vitro in the presence of NEDD4L.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant UBE2H protein, His Tag (CAT#: V2LY-0526-LY7499)

Related Products

Human Recombinant UBE2H protein, His Tag (CAT#: V2LY-0526-LY7499)

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Mouse Anti-UBE2H Recombinant Antibody (3C4-1A2) (CAT#: CBMAB-U0111-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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