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Mouse Anti-UBE2K Monoclonal Antibody (701316) (CBMAB-1679-YC)

Provided herein is a mouse monoclonal antibody against Human UBE2K. The antibody, clone 701316, can be used for immunoassay techniques, such as IHC and WB.
See all UBE2K antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
701316
Antibody Isotype
IgG2b
Application
IHC, WB

Basic Information

Immunogen
E. coli-derived recombinant human UBE2K/E2‑25K Ala2-Asn200.
Specificity
Human, Mouse
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ubiquitin Conjugating Enzyme E2 K
Introduction
UBE2K interacts with RING finger proteins and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for UBE2K include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.
Entrez Gene ID
Human3093
Mouse53323
UniProt ID
HumanP61086
MouseP61087
Alternative Names
LIG; HIP2; HYPG; E2-25K
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
Biological Process
Biological Process cellular response to interferon-beta Source:UniProtKB1 Publication
Biological Process free ubiquitin chain polymerization Source:UniProtKB1 Publication
Biological Process positive regulation of peptidyl-threonine phosphorylation Source:UniProtKB1 Publication
Biological Process positive regulation of tumor necrosis factor-mediated signaling pathway Source:FlyBase1 Publication
Biological Process positive regulation of type I interferon-mediated signaling pathway Source:UniProtKB1 Publication
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process regulation of proteasomal ubiquitin-dependent protein catabolic process Source:Ensembl
Biological Process ubiquitin-dependent protein catabolic process Source:ProtInc1 Publication
Cellular Location
Cytoplasm
PTM
Sumoylation at Lys-14 impairs catalytic activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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