Mouse Anti-UBE2S Recombinant Antibody (3D5) (CBMAB-A9728-LY)

Name: Mouse Anti-UBE2S Recombinant Antibody (3D5)
SKU: CBMAB-A9728-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

3D5

Application

WB, ELISA

Immunogen

UBE2S (AAH04236, 1 a.a. ~ 222 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ubiquitin Conjugating Enzyme E2 S

Introduction

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq]

Entrez Gene ID

27338

UniProt ID

Q16763

Alternative Names

E2-EPF; E2EPF; EPF5

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338).
Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151).
Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549).
In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).

Biological Process

Biological Process anaphase-promoting complex-dependent catabolic process Source:UniProtKB2 Publications
Biological Process cell division Source:UniProtKB-KW
Biological Process exit from mitosis Source:UniProtKB1 Publication
Biological Process free ubiquitin chain polymerization Source:UniProtKB1 Publication
Biological Process positive regulation of ubiquitin protein ligase activity Source:UniProtKB2 Publications
Biological Process protein K11-linked ubiquitination Source:UniProtKB3 Publications
Biological Process protein K27-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K29-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein modification process Source:ProtInc1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:GO_Central1 Publication

Cellular Location

anaphase-promoting complex
cytosol
nucleoplasm
nucleus

PTM

Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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