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Mouse Anti-UPF3A Recombinant Antibody (2E2) (CBMAB-C4698-CN)

This product is a Mouse antibody that recognizes UPF3A. The antibody 2E2 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all UPF3A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E2
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa 274-375 from human UPF3A (AAH23569) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
UPF3 regulator of nonsense transcripts homolog A (yeast)
Introduction
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
Entrez Gene ID
65110
UniProt ID
Q9H1J1
Alternative Names
UPF3A, Regulator Of Nonsense Mediated MRNA Decay; Up-Frameshift Suppressor 3 Homolog A; Nonsense MRNA Reducing Factor 3A; RENT3A; HUpf3; UPF3;
Function
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
Biological Process
Biological Process in utero embryonic development Source:Ensembl
Biological Process mRNA transport Source:UniProtKB-KW
Biological Process negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source:Ensembl
Biological Process positive regulation of translation Source:UniProtKB1 Publication
Biological Process spermatogenesis Source:Ensembl
Cellular Location
Nucleus
Cytoplasm
Shuttling between the nucleus and the cytoplasm.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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