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Rabbit Anti-USP25 Recombinant Antibody (CBXU-024) (CBMAB-U0140-CQ)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
CBXU-024
Application
WB, FC
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.393 mg/mL
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
ubiquitin specific peptidase 25
Introduction
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Among its related pathways are Metabolism of proteins and Ubiquitin-Proteasome Dependent Proteolysis. Gene Ontology (GO) annotations related to this gene include peptidase activity and thiol-dependent ubiquitin-specific protease activity.
Entrez Gene ID
Human29761
Mouse30940
Rat304150
UniProt ID
HumanQ9UHP3
MouseP57080
RatD4ACD3
Alternative Names
USP25; ubiquitin specific peptidase 25; ubiquitin specific protease 25; ubiquitin carboxyl-terminal hydrolase 25; USP21; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; ubiquitin-specific-processing protease 25
Function
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
Biological Process
Biological Process negative regulation of ERAD pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process protein deubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process protein K48-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein modification process Source:ProtInc1 Publication
Biological Process proteolysis Source:ProtInc1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Cytoplasm
Isoform USP25m
Cytoplasm
Nucleus
Some transient punctuate nuclear location in myotubes during myocyte development.
PTM
Acetylated.
Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-USP25 Recombinant Antibody (CBXU-047) (CAT#: CBMAB-U0162-CQ)

Mouse Anti-USP25 Recombinant Antibody (CBXU-090) (CAT#: CBMAB-U0190-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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