Human Recombinant USP25 protein, GST Tag (V2LY-0526-LY7566)

Name: Human Recombinant USP25 protein, GST Tag
SKU: V2LY-0526-LY7566
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant USP25 protein, GST Tag consist of Amino Acid: 162-677 and predicts a molecular mass of 85 kDa.

Molecule Mass

85 kDa

Sequence

Amino Acid: 162-677

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 25

Function

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

Biological Process

Biological Process negative regulation of ERAD pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process protein deubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process protein K48-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein K63-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein modification process Source:ProtInc1 Publication
Biological Process proteolysis Source:ProtInc1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Cytoplasm
Isoform USP25m
Cytoplasm
Nucleus
Some transient punctuate nuclear location in myotubes during myocyte development.

PTM

Acetylated.
Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-USP25 Recombinant Antibody (CBXU-047) (CAT#: CBMAB-U0162-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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