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Rabbit Anti-USP28 Recombinant Antibody (CBXU-027) (CBMAB-U0143-CQ)

This product is a rabbit antibody that recognizes USP28. The antibody CBXU-027 can be used for immunoassay techniques such as: WB.
See all USP28 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBXU-027
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ubiquitin specific peptidase 28
Introduction
The protein encoded by this gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of this gene is seen in several cancers.
Entrez Gene ID
Human57646
Mouse235323
Rat315639
UniProt ID
HumanQ96RU2
MouseQ5I043
RatD3ZJ96
Alternative Names
USP28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin carboxyl-terminal hydrolase 28; KIAA1515; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin-specific-processing protease 28
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
Biological Process
Biological Process cell population proliferation Source:UniProtKB1 Publication
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process cellular response to UV Source:UniProtKB1 Publication
Biological Process DNA damage checkpoint signaling Source:UniProtKB1 Publication
Biological Process DNA repair Source:UniProtKB-KW
Biological Process intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:UniProtKB3 Publications
Biological Process Ras protein signal transduction Source:UniProtKB1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process response to ionizing radiation Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Nucleus, nucleoplasm
PTM
Degraded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR (PubMed:16901786).
Phosphorylated by PRKD1 (PubMed:24623306).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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