Human Recombinant USP28, Active protein, GST Tag (V2LY-0526-LY7567)

Name: Human Recombinant USP28, Active protein, GST Tag
SKU: V2LY-0526-LY7567
Rating: 5 (1 reviews)

See all USP28 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant USP28, Active protein, GST Tag consist of Amino Acid: 155-675 and predicts a molecular mass of 85 kDa.

Molecule Mass

85 kDa

Sequence

Amino Acid: 155-675

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 28

Function

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).

Biological Process

Biological Process cell population proliferation Source:UniProtKB1 Publication
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process cellular response to UV Source:UniProtKB1 Publication
Biological Process DNA damage checkpoint signaling Source:UniProtKB1 Publication
Biological Process DNA repair Source:UniProtKB-KW
Biological Process intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:UniProtKB3 Publications
Biological Process Ras protein signal transduction Source:UniProtKB1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process response to ionizing radiation Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Nucleus, nucleoplasm

PTM

Degraded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR (PubMed:16901786).
Phosphorylated by PRKD1 (PubMed:24623306).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant USP28, Active protein, GST Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-USP28 Recombinant Antibody (CBXU-026) (CAT#: CBMAB-U0142-CQ)

Rabbit Anti-USP28 Recombinant Antibody (CBXU-027) (CAT#: CBMAB-U0143-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry