Human Anti-USP6 Recombinant Antibody (
3C3G2) (V2LY-0725-LY180)

Name: Human Anti-USP6 Recombinant Antibody (3C3G2)
SKU: V2LY-0725-LY180
Rating: 5 (1 reviews)

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Tested Data

Human Anti-USP6 Recombinant Antibody (3C3G2) (V2LY-0725-LY180) in WB

Western Blot Positive WB detected in: USP6 antibody at 1:1000 Lane 1: MCF-7 whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: HEK293 whole cell lysate Lane 6: Hela whole cell lysateSecondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 89, 122, 159 KDa Observed band size: 89, 122, 159 KDa Exposure time: 1min

Human Anti-USP6 Recombinant Antibody (3C3G2) (V2LY-0725-LY180) in IF

Immunofluorescence staining of HeLa cells with V2LY-0725-LY180 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Datasheet

SDS

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Basic Information

Host Animal

Human

Clone

3C3G2

Application

ELISA, WB, IF

Immunogen

Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348-535AA).

Host Species

Human

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:5,000
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 6 (Tre-2 oncogene)

Entrez Gene ID

9098

UniProt ID

P35125

Function

Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.

Biological Process

Biological Process activation of GTPase activity Source:GO_Central1 Publication
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process protein modification process Source:UniProtKB1 Publication
Biological Process regulation of vesicle-mediated transport Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Cell membrane
Cytoplasm
Endosome
Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.

Involvement in disease

A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).

PTM

Monubiquitinated; ubiquitination is calmodulin and calcium dependent.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-USP6 Recombinant Antibody (CBXU-104) (CAT#: CBMAB-U0200-CQ)

Mouse Anti-USP6 (aa 2-90) Recombinant Antibody (1F5) (CAT#: CBMAB-U0032-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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