Mouse Anti-VAPA Recombinant Antibody (4C12) (CBMAB-A9852-LY)

Name: Mouse Anti-VAPA Recombinant Antibody (4C12)
SKU: CBMAB-A9852-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

4C12

Application

WB, ELISA

Immunogen

VAPA (AAH02992, 1 a.a. ~ 242 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa

Introduction

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Entrez Gene ID

9218

UniProt ID

Q9P0L0

Alternative Names

MGC3745; VAP-33; VAP-A; VAP33; hVAP-33

Function

Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites (PubMed:25447204).
The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:25447204).
With OSBPL3, may regulate ER morphology (PubMed:16143324).
May play a role in vesicle trafficking (PubMed:11511104, PubMed:19289470).

Biological Process

Biological Process cell death Source:UniProtKB1 Publication
Biological Process ceramide transport Source:ComplexPortal1 Publication
Biological Process COPII-coated vesicle budding Source:UniProtKB1 Publication
Biological Process endoplasmic reticulum to Golgi vesicle-mediated transport Source:UniProtKB1 Publication
Biological Process membrane fusion Source:ProtInc1 Publication
Biological Process negative regulation by host of viral genome replication Source:AgBase1 Publication
Biological Process neuron projection development Source:UniProtKB1 Publication
Biological Process phospholipid transport Source:ComplexPortal1 Publication
Biological Process positive regulation by host of viral genome replication Source:AgBase1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process protein localization to endoplasmic reticulum Source:UniProtKB1 Publication
Biological Process sphingomyelin biosynthetic process Source:ComplexPortal1 Publication
Biological Process sterol transport Source:ComplexPortal1 Publication
Biological Process viral release from host cell Source:AgBase1 Publication

Cellular Location

Endoplasmic reticulum membrane
Cell membrane
Cell junction, tight junction
Nucleus membrane
Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.

Topology

Cytoplasmic: 2-227
Helical: 228-248

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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