Mouse Anti-VPS25 Recombinant Antibody (4D6-2D4) (CBMAB-V0007-YC)

Name: Mouse Anti-VPS25 Recombinant Antibody (4D6-2D4)
SKU: CBMAB-V0007-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

4D6-2D4

Application

ELISA, WB

Immunogen

MGC10540 (aa H06282, aa 1-176) full-length recombinant protein with GST tag. Immunogen sequence: MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF

Specificity

Human

Antibody Isotype

IgG1, к

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-176

More Infomation

Target

Full Name

vacuolar protein sorting 25 homolog (S. cerevisiae)

Introduction

VPS25 is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). The complex functions in sorting of ubiquitinated membrane proteins during endocytosis.

Entrez Gene ID

84313

UniProt ID

Q9BRG1

Alternative Names

Vacuolar Protein Sorting 25 Homolog; Dermal Papilla-Derived Protein 9; ELL-Associated Protein Of 20 KDa; ESCRT-II Complex Subunit VPS25; DERP9; EAP20

Function

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Biological Process

Biological Process macroautophagy Source:ParkinsonsUK-UCL1 Publication
Biological Process membrane fission Source:ComplexPortal1 Publication
Biological Process multivesicular body assembly Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of epidermal growth factor-activated receptor activity Source:UniProtKB1 Publication
Biological Process protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source:GO_Central1 Publication

Cellular Location

Cytoplasm
Endosome membrane
Nucleus, nucleoplasm
Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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