Mouse Anti-WDR77 Recombinant Antibody (
7D5) (V2LY-0725-LY325)

Name: Mouse Anti-WDR77 Recombinant Antibody (7D5)
SKU: V2LY-0725-LY325
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

7D5

Application

ELISA, WB, ICC

Immunogen

Purified recombinant Human WDR77 protein fragments expressed in E.coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
ICC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

WD Repeat Domain 77

Entrez Gene ID

79084

UniProt ID

Q9BQA1

Function

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452).
This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334).

Biological Process

Biological Process epithelial cell proliferation involved in prostate gland development Source:Ensembl
Biological Process histone H4-R3 methylation Source:ComplexPortal1 Publication
Biological Process negative regulation of epithelial cell proliferation involved in prostate gland development Source:Ensembl
Biological Process oocyte axis specification Source:GO_Central1 Publication
Biological Process positive regulation of cell population proliferation Source:Ensembl
Biological Process positive regulation of mRNA splicing, via spliceosome Source:ComplexPortal1 Publication
Biological Process regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development Source:Ensembl
Biological Process spliceosomal snRNP assembly Source:ComplexPortal1 Publication

Cellular Location

Nucleus
Cytoplasm
Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors (PubMed:17437848).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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