Rabbit Anti-WFDC2 Recombinant Antibody (
17D2) (V2LY-0725-LY1541)

Name: Rabbit Anti-WFDC2 Recombinant Antibody (17D2)
SKU: V2LY-0725-LY1541
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-WFDC2 Recombinant Antibody (17D2) (V2LY-0725-LY1541) in WB

Western Blot
Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate
All lanes: HE4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

Rabbit Anti-WFDC2 Recombinant Antibody (17D2) (V2LY-0725-LY1541) in FC

Overlay Peak curve showing MCF-7 cells stained with V2LY-0725-LY1541 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

17D2

Application

ELISA, WB, FC

Immunogen

A synthesized peptide derived from Human WFDC2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

WAP Four-Disulfide Core Domain 2

Entrez Gene ID

10406

UniProt ID

Q14508

Function

Broad range protease inhibitor.

Biological Process

Biological Process antibacterial humoral response Source:GO_Central1 Publication
Biological Process innate immune response Source:GO_Central1 Publication
Biological Process proteolysis Source:ProtInc1 Publication
Biological Process spermatogenesis Source:ProtInc1 Publication

Cellular Location

Secreted

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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