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Mouse Anti-XAB2 Recombinant Antibody (1D1-1A9) (CBMAB-A9958-LY)

The product is antibody recognizes XAB2. The antibody 1D1-1A9 immunoassay techniques such as: WB, ELISA.
See all XAB2 antibodies
Published Data
Fig. 1 HL60 cells were transfected with sense siRNAs of XAB2 gene (XAB2 siRNA ss1 or ss2) or with control nonsense siRNA (Control siRNA) using Nucleofector. Cell lysates were resolved by 10% SDS-PAGE and immunoblotted with anti-XAB2 mAb, followed by stripping and reprobing with antitubulin mAb.
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Fig. 2 In the absence of ATRA, nuclear extracts were prepared from HL60 cells and immunoprecipitation (IP) assays were conducted with anti-XAB2 antibody or control immunoglobulin (cIgG). Immunoprecipitation complexes, along with 10% input of nuclear extract, were then separated by 4% to 20% SDS-PAGE, followed by Western blotting with indicated antibodies.
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Fig. 3 HL60 cells were treated with ATRA (1 Amol/L) or DMSO vehicle for 24 h and submitted to analysis using confocal laser microscopy.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D1-1A9
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
XAB2 (AAH07208.1, 1 a.a. ~ 855 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
XPA binding protein 2
Entrez Gene ID
56949
UniProt ID
Q9HCS7
Alternative Names
DKFZp762C1015; HCNP; HCRN; NTC90; SYF1
Function
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346).
Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing (PubMed:10944529, PubMed:17981804).
Biological Process
Biological Process blastocyst development Source:Ensembl
Biological Process cerebral cortex development Source:Ensembl
Biological Process DNA-templated transcription Source:UniProtKB1 Publication
Biological Process generation of catalytic spliceosome for first transesterification step Source:GO_Central1 Publication
Biological Process mRNA splicing, via spliceosome Source:UniProtKB1 Publication
Biological Process transcription-coupled nucleotide-excision repair Source:UniProtKB1 Publication
Cellular Location
Nucleus
Detected in the splicing complex carrying pre-mRNA.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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