Mouse Anti-XRCC3 Recombinant Antibody (10F1/6) (CBMAB-X0191-YC)

Name: Mouse Anti-XRCC3 Recombinant Antibody (10F1/6)
SKU: CBMAB-X0191-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

10F1/6

Application

WB, IHC, ICC, IF

Immunogen

His-tagged human XRCC3, over-expressed in E. coli [UniProt# O43542]

Specificity

Human

Antibody Isotype

IgG1, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

X-ray repair complementing defective repair in Chinese hamster cells 3

Introduction

XRCC3 is a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. A rare microsatellite polymorphism in XRCC3 is associated with cancer in patients of varying radiosensitivity.

Entrez Gene ID

7517

UniProt ID

O43542

Alternative Names

CMM6

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

Biological Process

Biological Process cellular response to DNA damage stimulus Source:ProtInc1 Publication
Biological Process DNA recombination Source:ProtInc1 Publication
Biological Process DNA repair Source:BHF-UCL1 Publication
Biological Process double-strand break repair via homologous recombination Source:UniProtKB1 Publication
Biological Process double-strand break repair via synthesis-dependent strand annealing Source:GO_Central1 Publication
Biological Process interstrand cross-link repair Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of mitotic cell cycle spindle assembly checkpoint Source:UniProtKB1 Publication
Biological Process regulation of centrosome duplication Source:UniProtKB1 Publication
Biological Process resolution of mitotic recombination intermediates Source:UniProtKB1 Publication
Biological Process response to organic substance Source:Ensembl
Biological Process t-circle formation Source:BHF-UCL1 Publication
Biological Process telomere maintenance via recombination Source:BHF-UCL1 Publication
Biological Process telomere maintenance via telomere trimming Source:BHF-UCL1 Publication
Biological Process telomeric loop disassembly Source:BHF-UCL1 Publication

Cellular Location

Nucleus
Cytoplasm
Cytoplasm, perinuclear region
Mitochondrion
Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.

Involvement in disease

Breast cancer (BC):
A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Melanoma, cutaneous malignant 6 (CMM6):
A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but may also involve other sites.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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