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Rabbit Anti-YTHDC1 Recombinant Antibody (E2P2I) (CBMAB-CP3046-LY)

The product is antibody recognizes YTHDC1. The antibody E2P2I immunoassay techniques such as: WB,IP.
See all YTHDC1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
E2P2I
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly11 of human YTHDC1 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
YTH Domain Containing 1
Introduction
YTHDC1 (YTH Domain Containing 1) is a Protein Coding gene. Among its related pathways are Chromatin Regulation / Acetylation. Gene Ontology (GO) annotations related to this gene include N6-methyladenosine-containing RNA binding.
Entrez Gene ID
Human91746
Mouse231386
Rat170956
Monkey709717
UniProt ID
Human96MU7
MouseE9Q5K9
RatQ9QY02
MonkeyI2CYY0
Alternative Names
YTH Domain Containing 1; Splicing Factor YT521; YT521-B; YT521; YTH Domain-Containing Protein 1; Putative Splicing Factor YT521; Splicing Factor YT521-B; KIAA1966;
Function
Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:25242552, PubMed:26318451, PubMed:26876937, PubMed:28984244).
M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:25242552, PubMed:26318451).
Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937).
Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937).
In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937).
May also regulate alternative splice site selection (PubMed:20167602).
Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).
Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity).
Also recognizes and binds m6A on other RNA molecules (PubMed:27602518).
Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518).
Also recognizes and binds m6A-containing single-stranded DNA (PubMed:32663306).
Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity).
Biological Process
Biological Process dosage compensation by inactivation of X chromosome Source:UniProtKB1 Publication
Biological Process in utero embryonic development Source:Ensembl
Biological Process mRNA alternative polyadenylation Source:Ensembl
Biological Process mRNA export from nucleus Source:UniProtKB1 Publication
Biological Process mRNA splice site selection Source:UniProtKB1 Publication
Biological Process mRNA splicing, via spliceosome Source:GO_Central1 Publication
Biological Process post-transcriptional regulation of gene expression Source:UniProtKB
Biological Process primary follicle stage Source:Ensembl
Biological Process regulation of alternative mRNA splicing, via spliceosome Source:GO_Central1 Publication
Biological Process regulation of mRNA splicing, via spliceosome Source:UniProtKB2 Publications
Biological Process spermatogenesis Source:Ensembl
Cellular Location
Nucleus
Nucleus speckle
Localizes to a novel subnuclear structure, the YT bodies.
PTM
Tyrosine phosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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